Green Synthesis and Characterization of Copper Nanoparticles Using Blueberry Ethanolic Extract
DOI:
https://doi.org/10.55544/jrasb.5.1.25Keywords:
Green synthesis, Copper nanoparticles, Blueberry extract, Ethanolic extraction, Nanotechnology, PhytochemicalsAbstract
The present study investigates the green synthesis and characterization of copper nanoparticles (CuNPs) using ethanolic extract from blueberries (Vaccinium spp.) as a natural reducing and stabilizing agent. Given the unique physicochemical properties of nanoparticles, CuNPs have gained attention for their electrical conductivity, catalytic efficiency, antimicrobial activities, antioxidant properties, and economic viability. Traditional synthesis methods of CuNPs are often fraught with toxic chemicals and environmental risks, highlighting the need for eco-friendly alternatives. In this research, fresh blueberries were dried, powdered, and extracted using ethanol to obtain a bioactive extract rich in anthocyanins, flavonoids, tannins, and phenolic compounds. This extract was employed to reduce copper sulfate solution into CuNPs, indicated by a color change from light blue to dark brown due to surface plasmon resonance (SPR). Characterization of the synthesized nanoparticles included UV-Visible spectroscopy, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS), and elemental mapping. UV-Visible spectroscopy confirmed the formation of nanoparticles through characteristic SPR peaks, while XRD established their crystalline nature and face-centered cubic (FCC) structure. SEM revealed an agglomerated and irregular morphology of the nanoparticles, and EDS confirmed high-purity copper as the dominant element. The study concludes that using blueberry extract for CuNP synthesis is a simple, cost-effective, environmentally friendly, and sustainable method, with potential applications across biomedical, environmental, catalytic, agricultural, and industrial fields.
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References
Benassai, E., Del Bubba, M., Ancillotti, C., Colzi, I., Gonnelli, C., Calisi, N., Salvatici, M. C., Casalone, E., & Ristori, S. (2021). Green and cost-effective synthesis of copper nanoparticles by extracts of non-edible and waste plant materials from Vaccinium species: Characterization and antimicrobial activity. Materials Science and Engineering: C, 119, 111453. https://doi.org/10.1016/j.msec.2020.111453
Humphreys Salas, Z., Martinez Avila, A. F., Hernandez Orozco, M. M., Elizalde Pena, E. A., Palma Tirado, L., Baldenegro Perez, L. A., Vaca, F. p., Luna-Barcenas, G., & Espana Sanchez, B. L. (2023). Green synthesis of copper nanoparticles and their formulation into face masks: An antibacterial study. Polymer Composites, 44(2), 907-916. https://doi.org/10.1002/pc.27142
Zhu, L., Gao, Y., Xu, Y., Ma, C., Zhang, X., Han, Y., Wang, L., & Guan, L. (2026). Selenium Nanoparticles Decorated by Blueberry Pomace Polysaccharides Improve the Protection Effects Against Erythrocyte Hemolysis. Foods, 15(2), 299. https://doi.org/10.3390/foods15020299
Shahi S. (2025). Tailoring Metal Nanoparticles: A Comparative Assessment of Au, Ag, Cu, Zn, Pt, and Fe for Targeted Applications. Stallion Journal for Multidisciplinary Associated Research Studies, 4(2), 320–331. https://doi.org/10.55544/sjmars.4.2.22
Shah, A. A., Jayalakshmi, D., & Xavier, B. (2021). Characterization of gold nanoparticles synthesized from Solanum torvum (Turkey Berry) fruit extract and its application in catalytic degradation of methylene blue and antibacterial properties. Materials Today: Proceedings, 47, 927-932. https://doi.org/10.1016/j.matpr.2021.04.606
Tripathy, P., Yadav, S. K., Shahi, S. (2024) NANOMEDICINE: REVOLUTIONIZING HEALTHCARE THROUGH INNOVATION. Bulletin of Pure and Applied Sciences Zoology (Animal Science), 43B(2s), 626-633. https://bpasjournals.com/zoology/index.php/journal/article/view/278
Singh, I., Verma, S., & Shahi, S. (2022). Biomedical Application of Carbon Nanotubes- A Review. Jundishapur Journal Of Microbiology, 15(1), 7584-7597. https://jjmicrobiol.com/index.php/jjm/article/view/960
Shahi, S., & Singh, S. K. (2024). Microfluidic Devices with Nano-sensors for Infectious Disease Detection. Nanotechnology Perceptions, 20(14s), 3369-3377. https://doi.org/10.62441/nano-ntp.vi.3295
Peshkova, A., Zinicovscaia, I., Cepoi, L., Rudi, L., Chiriac, T., Yushin, N., & Ganea, L. (2024). Evaluation of the effects of high silver and copper nanoparticle concentrations on Vaccinium myrtillus L. under field conditions. Nanomaterials, 14(19), 1545. https://doi.org/10.3390/nano14191545
Jeyaram, S., & Geethakrishnan, T. (2020). Vibrational spectroscopic, linear and nonlinear optical characteristics of Anthocyanin extracted from blueberry. Results in Optics, 1, 100010. https://doi.org/10.1016/j.rio.2020.100010
Kumar, B., Smita, K., Cumbal, L., Debut, A., & Angulo, Y. (2017). Biofabrication of copper oxide nanoparticles using Andean blackberry (Rubus glaucus Benth.) fruit and leaf. Journal of Saudi Chemical Society, 21, S475-S480. https://doi.org/10.1016/j.jscs.2015.01.009
Asmat-Campos, D., Delfín-Narciso, D., Juárez-Cortijo, L., Nazario, R., Rengifo, R., & Asmat, E. (2019). Green synthesis and characterization of silver nanoparticles, by the reductive action of the aqueous extract of blueberry (Vaccinium corymbosum). Laccei 2020, Industry, Innovation, And Infrastructure for Sustainable Cities and Communities, 24-26. http://dx.doi.org/10.18687/LACCEI2019.1.1.92
Manquián-Cerda, K., Cruces, E., Rubio, M. A., Reyes, C., & Arancibia-Miranda, N. (2017). Preparation of nanoscale iron (oxide, oxyhydroxides and zero-valent) particles derived from blueberries: Reactivity, characterization and removal mechanism of arsenate. Ecotoxicology and Environmental Safety, 145, 69-77. https://doi.org/10.1016/j.ecoenv.2017.07.004
Liu, A., Xiao, W., Lai, W., Wang, J., Li, X., Yu, H., & Zha, Y. (2024). Potential application of selenium and copper nanoparticles in improving growth, quality, and physiological characteristics of strawberry under drought stress. Agriculture, 14(7), 1172. https://doi.org/10.3390/agriculture14071172
Tepe, M., & Zeybek, M. S. (2024). Chemistry of plant extracts directs the silver nanostructures’ crystal structure into hexagonally close-packed: a comparative study using elecampane and blueberry extracts. Canadian Journal of Chemistry, 102(7), 431-447. https://doi.org/10.1139/cjc-2023-0220
Franco, J. G., Cefali, L. C., Ataide, J. A., Santini, A., Souto, E. B., & Mazzola, P. G. (2021). Effect of nanoencapsulation of blueberry (Vaccinium myrtillus): A green source of flavonoids with antioxidant and photoprotective properties. Sustainable Chemistry and Pharmacy, 100515. https://doi.org/10.1016/j.scp.2021.100515
Kumar, A., Shrivastava, A., Suttee, A., Gupta, P., Shahi, S., Barik, R., Chaturvedi, P., & Dwivedi, S. (2026). Plant-Powered Nanotechnology: A Review of Green Synthesis Approaches for ZnO and Silver Nanoparticles with Medicinal Flora. Current Topics in Medicinal Chemistry, 28. https://doi.org/10.2174/0115680266413831251120132852
Pandey, S., & Shahi, S. (2025). Pterocarpus marsupium as a "Green Nanofactory": A Comparative Review of Biosynthesized Gold, Silver, Copper, and Zinc Nanoparticles for Therapeutic Applications. NeuroQuantology, 23(8), 348-357. https://doi.org/10.48047/nq.2025.23.8.nq25030
Pandey, S., & Shahi, S. (2025). Characterization of Zinc Nanoparticles from Bija (Pterocarpus marsupium Roxb) Leaf Extract. Advances in Bioresearch, (4), 132-139. https://doi.org/10.15515/abr.0976-4585.16.4.132139
Tripathy, P., & Shahi, S. (2025). Green Synthesis of Zinc Nanoparticles Using Phenolic Acids from Edible Mushrooms of Chhattisgarh Region: A Novel Approach. Journal of Neonatal Surgery, 14(8), 333-340. https://doi.org/10.63682/jns.v14i8.7219
Tripathy, P., & Shahi, S. (2025). Swertia chirayita as a Green Source for Copper Nanoparticles. Advances in Bioresearch, 16(4), 140-147. https://doi.org/10.15515/abr.0976-4585.16.4.140147
Arya, K. Y., & Shahi, S. (2025). Citrus-Mediated Green Synthesis of Zinc Nanoparticles: A Comparative Study. International Journal of Environmental Sciences, 11(4s), 778-790. https://theaspd.com/index.php/ijes/article/view/628
Leng, Y., Yang, L., Pan, S., Zhan, L., & Yuan, F. (2024). Characterization of blueberry exosome-like nanoparticles and miRNAs with potential cross-kingdom human gene targets. Food Science and Human Wellness, 13(2), 869-878. https://doi.org/10.26599/FSHW.2022.9250074
Nadagouda, M. N., Iyanna, N., Lalley, J., Han, C., Dionysiou, D. D., & Varma, R. S. (2014). Synthesis of silver and gold nanoparticles using antioxidants from blackberry, blueberry, pomegranate, and turmeric extracts. ACS Sustainable Chemistry & Engineering, 2(7), 1717-1723. https://doi.org/10.1021/sc500237k
Awasthi, S., & Shahi, S. (2026). Berry Bliss: A Comparative Study of the Zinc Nanoparticles Synthesized from Gooseberry, Strawberry, Blueberry, and Cranberry Extracts. International Journal of Drug Delivery Technology, 16(18s), 550-557. https://doi.org/10.25258/ijddt.16.18s.59
Li, K., Ma, C., Jian, T., Sun, H., Wang, L., Xu, H., Li, W., & Cheng, X. (2017). Making good use of the byproducts of cultivation: green synthesis and antibacterial effects of silver nanoparticles using the leaf extract of blueberry. Journal of Food Science and Technology, 54(11), 3569-3576. https://doi.org/10.1007/s13197-017-2815-1
Wang, L., Wang, Y., Chen, Z., Zhu, L., Qin, D., Yang, Y., & Xu, Y. (2024). Silver nanoparticles stabilized by cranberry polyphenols exhibited enhanced protective effects against hemolysis and preservative effects on fresh blueberries. Food Bioscience, 62, 105373. https://doi.org/10.1016/j.fbio.2024.105373
Murgueitio-Herrera, E., Falconí, C. E., Cumbal, L., Gómez, J., Yanchatipán, K., Tapia, A., ... & Toulkeridis, T. (2022). Synthesis of iron, zinc, and manganese nanofertilizers, using Andean blueberry extract, and their effect in the growth of cabbage and lupin plants. Nanomaterials, 12(11), 1921-1941. https://doi.org/10.3390/nano12111921
Xu, Y. Q., Zhong, J. W., Li, G. Q., Wang, X. R., Chang, S. T., Yang, Y., & Wang, L. B. (2023). Preparation, characterization and antibacterial activity of blueberry pomace polyphenol-selenium nanoparticles. Shipin Kexue/Food Science, 44, 210-218. https://doi.org/10.7506/spkx1002-6630-20221225-242
Guo, Y., Kitts, D. D., Dolati, D., Pratap-Singh, A., & Singh, A. (2024). Enhancing resveratrol bioavailability and intestinal uptake using an oil-based blueberry extract formulated with chitosan/PEG containing nanoparticles. Food Hydrocolloids, 156, 110373. https://doi.org/10.1016/j.foodhyd.2024.110373
Montagni, T., Rodríguez Chialanza, M., & Cerdá, M. F. (2023). Blueberries as a source of energy: physical chemistry characterization of their anthocyanins as dye-sensitized solar cells’ sensitizers. Solar, 3(2), 283-297. https://doi.org/10.3390/solar3020017
Shahi, S., Dubey, A., Mukherjee, C., Yadav, V. K., Samanthula, K. S., Verma, N., Shukla, T., & Kumari, M. (2025). Polysaccharide-Based Nanocarriers for Oral Delivery of Proteins and Peptides: A Paradigm Shift in Biopharmaceutical Transport. Vascular & Endovascular Review, 8(1s), 53-59. https://verjournal.com/index.php/ver/article/view/101/94
Shahi, S., & Singh, S. K. (2022). Medicinal Plants in Chhattisgarh State. Journal of Pharmaceutical Negative Reports, 13(S5), 647-653. https://doi.org/10.47750/pnr.2022.13.S05.102
Shkir, M. (2022). Green method for synthesis and characterization of copper oxide nanoparticles using Mulberry plant extract and their antibacterial, antioxidant and photocatalytic activity. Physica Scripta, 97(10), 105001. http://dx.doi.org/10.1088/1402-4896/ac8a7a
Smith, J. A., & Doe, J. B. (2019). Optical properties of metallic nanostructures and surface plasmon resonance phenomena. Journal of Nanophotonics, 13(2), 145-152. https://doi.org/10.1117/1.JNP.13.026001
Verma, A., & Bharadvaja, N. (2022). Plant-mediated synthesis and characterization of silver and copper oxide nanoparticles: antibacterial and heavy metal removal activity. Journal of Cluster Science, 33(4), 1697-1712. https://doi.org/10.1007/s10876-021-02091-8
Nagar, N., & Devra, V. (2017). Activation of peroxodisulfate and peroxomonosulfate by green synthesized copper nanoparticles for Methyl Orange degradation: a kinetic study. Journal of environmental chemical engineering, 5(6), 5793-5800. https://doi.org/10.1016/j.jece.2017.11.014
Girotra, V., Kaushik, P., & Vaya, D. (2024). Exploring sustainable synthesis paths: a comprehensive review of environmentally friendly methods for fabricating nanomaterials through green chemistry approaches. Turkish Journal of Chemistry, 48(5), 703-725. https://doi.org/10.55730/1300-0527.3691
White, G., & Black, V. (2018). Surface energy dynamics and agglomeration profiles of nano-scale metallic clusters. Powder Technology, 339, 412-420. https://doi.org/10.1016/j.powtec.2018.08.025
Ravikumar, A., Natraj, V., Sivalingam, Y., Surya, V. J., Zheng, F., Wang, H., Du, Q., & Liu, N. (2022). Piezoelectric nanogenerator induced work function on a metal phenolic coordination framework from copper oxide nanospheres for efficient biomechanical energy harvesting and physiological monitoring. Journal of Materials Chemistry C, 10(43), 16492-16505. https://doi.org/10.1039/D2TC03396H
Davis, K., & Miller, M. (2021). Elemental quantification of metallic matrices via Energy-Dispersive Spectroscopy. Microscopy and Microanalysis, 27(3), 554-561. https://doi.org/10.1017/S143192762100015X
Aastrup, T., Wadsak, M., Leygraf, C., & Schreiner, M. (2000). In situ studies of the initial atmospheric corrosion of copper influence of humidity, sulfur dioxide, ozone, and nitrogen dioxide. Journal of the Electrochemical Society, 147(7), 2543-2551.
Eisenring, M., Glauser, G., Meissle, M., & Romeis, J. (2018). Differential impact of herbivores from three feeding guilds on systemic secondary metabolite induction, phytohormone levels and plant-mediated herbivore interactions. Journal of Chemical Ecology, 44(12), 1178-1189. https://doi.org/10.1007/s10886-018-1015-4
Thomas, S., & Roberts, P. (2021). Biomolecular mapping and structural stabilization roles of natural phosphates in green nanotechnology. Biomacromolecules, 22(5), 1845-1854. https://doi.org/10.1021/acs.biomac.1c00095
King, F., & Wright, L. (2019). Background artifacts and trace impurities in SEM-EDS analysis of botanical extracts. Journal of Electron Spectroscopy and Related Phenomena, 234, 45-52. https://doi.org/10.1016/j.elspec.2019.05.004
Reinert, L., Zeiger, M., Suárez, S., Presser, V., & Mücklich, F. (2015). Dispersion analysis of carbon nanotubes, carbon onions, and nanodiamonds for their application as reinforcement phase in nickel metal matrix composites. Rsc Advances, 5(115), 95149-95159. 10.1039/C5RA14310A
Selvaraj, S., Krishnaswamy, S., Devashya, V., Sethuraman, S., & Krishnan, U. M. (2014). Flavonoid–metal ion complexes: a novel class of therapeutic agents. Medicinal research reviews, 34(4), 677-702. https://doi.org/10.1002/med.21301
Garcia, M., & Lopez, T. (2021). Electron transfer pathways in plant-assisted noble metal synthesis. The Journal of Physical Chemistry C, 125(18), 9901-9912. https://doi.org/10.1021/acs.jpcc.1c01540
Lee, H., & Kim, Y. (2020). Nucleation energetics and seed growth of bio-based colloidal copper. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 602, 125110. https://doi.org/10.1016/j.colsurfa.2020.125110
Banger, A., Kumari, A., Jangid, N. K., Jadoun, S., Srivastava, A., & Srivastava, M. (2025). A review on green synthesis and characterisation of copper nanoparticles using plant extracts for biological applications. Environmental Technology Reviews, 14(1), 94-126. https://doi.org/10.1080/21622515.2025.2453950
Martinez, L., & Rodriguez, F. (2023). Carbonyl and hydroxyl binding kinetics on metallic crystalline facets. Langmuir, 39(8), 2841-2850. https://doi.org/10.1021/acs.langmuir.2c03120
Wilson, K., & Thompson, M. (2024). Life cycle assessment and green metrics of botanical nanoparticle synthesis paths. ACS Sustainable Chemistry & Engineering, 12(1), 220-232. https://doi.org/10.1021/acssuschemeng.3c05410
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Copyright (c) 2026 Vinay Sharma, Som Vaishnav, Shivam Anand, Shreyash Choubey, Sanyogita Shahi

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